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Fig. 7 | Cancer & Metabolism

Fig. 7

From: RHOF promotes Snail1 lactylation by enhancing PKM2-mediated glycolysis to induce pancreatic cancer cell endothelial–mesenchymal transition

Fig. 7

RHOF promotes EMT via inducing glycolysis and Snail1 lactylation in PC cell. A IP assay was applied to identify the interaction between Snail1 and Pan-Kla. B Western blot was taken to test Snail1 expression. C Immunofluorescence co-localization detection of Kla and Snail1. D IP assay was applied to detect the interaction between Snail1 and Pan-Kla under 2-DG treatment. E Western blot was taken to test Snail1 expression under 2-DG treatment. F Immunofluorescence co-localization detection of Kla and Snail1 under 2-DG treatment. G Scratch experiment was utilized to estimate cell migration. H Cell migation and invasion were estimate by Transwell assay (Magnification × 100). I Western blot was utilized to estimate the protein expression of E-cadherin, Vimentin, and N-cadherin. J The mechanism diagram of RHOF in regulating PC cell glycolysis and EMT. n = 3. Data were analyzed using the one‑way ANOVA (three group comparisons) or paired t-test (two group comparisons)

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Cancer & Metabolism

ISSN: 2049-3002