Fig. 4

LDL from BC patients induces a distinct macrophage polarization pattern. Primary human monocytes pooled from three healthy donors were differentiated with 20ng/ml M-CSF for 7 days and then treated for 24 h with 50 µg/ml LDL isolated from plasma of healthy women (n = 10) or BC patients (n = 10). Relative gene expression was measured by real-time PCR. P value was calculated using two-tailed Student’s t-test. ns– not significant, * (p < 0.05), ** (p < 0.01)