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Fig. 5 | Cancer & Metabolism

Fig. 5

From: ETV4/ALYREF-mediated glycolytic metabolism through PKM2 enhances resistance to ferroptosis and promotes the development of intrahepatic cholangiocarcinoma

Fig. 5

ETV4 targets and regulates ALYREF to promote ICC cell proliferation. (A) ETV4 was positively correlated with ALYREF. (B) The expression of ETV4, ALYREF, and PKM2 in ICC cells detected by WB. (C) ICC cell proliferation assessed by CCK-8 assay. (D) ICC cell proliferation detected by EdU assay (Magnification: ×50, scale bar = 200 μm). (E) ICC cell migration examined by Transwell assay (Magnification: ×100, scale bar = 100 μm). (F) Bioinformatic prediction of binding sites between ETV4 and ALYREF, and ALYREF’s motif diagram. (G) ChIP validation of the binding of ETV4 and ALYREF to the promoter. *P < 0.05; **P < 0.01; ***P < 0.001; n = 3

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Cancer & Metabolism

ISSN: 2049-3002