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Fig. 2 | Cancer & Metabolism

Fig. 2

From: ETV4/ALYREF-mediated glycolytic metabolism through PKM2 enhances resistance to ferroptosis and promotes the development of intrahepatic cholangiocarcinoma

Fig. 2Fig. 2

ETV4 promotes ICC cell resistance to ferroptosis through PKM2-mediated glycolytic metabolism. (A) The expression levels of ETV4 and PKM2 were detected by WB. (B) ICC cell proliferation was assessed by CCK-8 assay. (C) ICC cell proliferation was detected by the EdU assay (Magnification: ×50, scale bar = 200 μm). (D) ICC cell migration was examined by Transwell assay (Magnification: ×100, scale bar = 100 μm). (E-G) Measurement of GLU, LD, and ATP content in ICC cells. (H) The expression of PDK1, LDHA, and GLUT1 assessed by WB. (I) CCK-8 assay was used to screen Erastin concentration. (J) ICC cell proliferation was assessed by CCK-8 assay. (K) Transwell assay was used to evaluate ICC cell migration after erastin induction (Magnification: ×100, scale bar = 100 μm). (L) FCM was used to measure ICC cell lipid ROS levels. (M) ELISA was used to detect MDA and Fe2+ levels in ICC cells. *P < 0.05; **P < 0.01; ***P < 0.001; n = 3

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Cancer & Metabolism

ISSN: 2049-3002