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Fig. 4 | Cancer & Metabolism

Fig. 4

From: TRIM22 governs tumorigenesis and protects against endometrial cancer-associated cachexia by inhibiting inflammatory response and adipose thermogenic activity

Fig. 4

TRIM22 OE blocked EC-induced browning of WAT. (A) Representative images of H&E and IHC-stained sections of IngWAT from xenograft mice subcutaneously injected PBS, scramble control and TRIM22 OE Ishikawa cells. (B) Statistical analysis of lipid aera in IngWAT from xenograft mice subcutaneously injected PBS, scramble control and TRIM22 OE Ishikawa cells in (A) using H&E staining. (C) Statistical analysis of UCP1 expression in IngWAT from xenograft mice subcutaneously injected PBS, scramble control and TRIM22 OE Ishikawa cells in (A) using IHC staining. (D) mRNA level of genes of IngWAT from xenograft mice subcutaneously injected PBS, scramble control and TRIM22 OE Ishikawa cells, including thermogenic genes, fatty acid oxidation related genes, lipolysis related genes, mitochondrial biogenic transcription factor. (E) mRNA level of gene of beige cell marker (TMEM26) in IngWAT from xenograft mice subcutaneously injected PBS, scramble control and TRIM22 OE Ishikawa cells. (F) mRNA level of gene of beige cell marker (CD137) in IngWAT from xenograft mice subcutaneously injected PBS, scramble control and TRIM22 OE Ishikawa cells. (G) mRNA level of gene of beige cell marker (TBX1) in IngWAT from xenograft mice subcutaneously injected PBS, scramble control and TRIM22 OE Ishikawa cells. (H) Mean OCR from Seahorse in IngWAT from xenograft mice subcutaneously injected PBS, scramble control and TRIM22 OE Ishikawa cells. Data were expressed as means ± SEM of three independent experiments. Scale bar, 50 μm. *p < 0.05, **p < 0.01, ***p < 0.001

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Cancer & Metabolism

ISSN: 2049-3002