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Fig. 2 | Cancer & Metabolism

Fig. 2

From: TRIM22 governs tumorigenesis and protects against endometrial cancer-associated cachexia by inhibiting inflammatory response and adipose thermogenic activity

Fig. 2

TRIM22 OE suppressed EC progression and prevented body weight loss induced by cachexia. (A) Ratio of Edu-positive scramble control and TRIM22 OE Ishikawa cells in (A). (B) Representative images of scramble control and TRIM22 OE Ishikawa cells that cultured in trans-well plates. (C) The average number of migration Ishikawa cells in (C). (D) The average number of invasion Ishikawa cells in (C). (E) Representative images showing Edu incorporation in scrambling control and TRIM22 KD RL-952 cells. (F) Ratio of Edu-positive RL-952 cells in (F). (G) Representative images of scramble control and TRIM22 KD RL-952 cells that cultured in trans-well plates. (H) The average number of migration RL-952 cells in (H). (I) The average number of invasion RL-952 cells in (H). (J) A subcutaneous tumor growth curve from scramble control and TRIM22 OE Ishikawa cells at indicated time points (n = 9 mice per group). (K) Representative images of xenograft tumors in (K) at day 27 (n = 9 mice per group). (L) Weights of xenograft tumors in (L) at day 27 (n = 9 mice per group). (M)Representative H&E staining and IHC staining of Ki67 in xenograft tumors derived from (L). (N) Statistical analysis of Ki67 expression in xenograft tumors derived from (L) using IHC staining. (O) Statistical analysis of lipid aera in xenograft tumors derived from (L) using H&E staining. (P) A body weight curve from xenograft mice subcutaneously injected PBS, scramble control and TRIM22 OE Ishikawa cells. (Q) Statistical analysis of body weight gain of xenograft mice subcutaneously injected PBS, scramble control and TRIM22 OE Ishikawa cells. (R) Statistical analysis of carcass weight of xenograft mice subcutaneously injected PBS, scramble control and TRIM22 OE Ishikawa cells. (S) Statistical analysis of the mass of BAT from xenograft mice subcutaneously injected PBS, scramble control and TRIM22 OE Ishikawa cells. (T) Statistical analysis of the mass of IngWAT from xenograft mice subcutaneously injected PBS, scramble control and TRIM22 OE Ishikawa cells. (U) Statistical analysis of the mass of GonWAT from xenograft mice subcutaneously injected PBS, scramble control and TRIM22 OE Ishikawa cells. Data were expressed as means ± SEM of three independent experiments. Scale bar, 50 μm. *p < 0.05, **p < 0.01, ***p < 0.001

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Cancer & Metabolism

ISSN: 2049-3002