Table 1 Media compositions used for culturing each cell line prior to seeding for an experiment
From: Ketomimetic nutrients remodel the glycocalyx and trigger a metabolic defense in breast cancer cells
| Â | MCF-10A | MCF-7 and MDA-MB-231 |
|---|---|---|
High glucose media (HG) | DMEM/F12 (3.15 g/L glucose) supplemented with heat-inactivated equine serum (5%), Penicillin–Streptomycin mixture (1%), insulin (0.01 mg/mL), hydrocortisone (o.5 µg/mL), EGF (0.2 µg/mL) and cholera toxin (0.1 µg/mL) | High-glucose (4.5 g/L) DMEM supplemented with heat-inactivated fetal bovine serum (10%), Penicillin–Streptomycin mixture (1%), and insulin (0.01 mg/mL) |
Low glucose media (LG) | Low-glucose (1g/L) DMEM mixed with Ham’s F12 (1:1) and supplemented with heat-inactivated equine serum (5%), Penicillin–Streptomycin mixture (1%), hydrocortisone (0.5 µg/mL), EGF (0.2 µg/mL) and cholera toxin (0.1 µg/mL) | Low-glucose (1g/L) DMEM supplemented with heat-inactivated fetal bovine serum (10%), and Penicillin–Streptomycin mixture (1%) |