Fig. 3

Glucose consumption and migratory propensity of cells in different nutrient media. A Glucose consumption patterns of MDA-MB-231, MCF-7 and MCF-10A cells in the respective nutrient media: high glucose (HG), low glucose (LG) and HBLG (hydroxybutyrate containing low glucose). Changes in the amount of glucose consumed by the cells after four days of culture are plotted with data from n = 3 and N = 2 being pooled together. GraphPad Prism was used for plotting the data with colored bars representing means and error bars representing SEM, two-tailed unpaired t-tests were performed for column-wise statistical analysis. B Cellular proliferation profiles of the three cell lines in the respective nutrient media. Symbols denote mean values and error bars denote SEM from N = 2 and n = 2. C Cellular migration profile of MDA-MB-231 cells in response to changes in nutrient media quantified using images of crystal violet stained cells that have migrated across transwell membrane. Images from two separate experiments (N = 2) were used with 8–10 different FOVs collected for each experiment. The percentage (%) occupied area was measured using ImageJ software; bar graph displays mean values with error bars denoting SEM. Representative images are shown in the right panel, scale bar = 50 µm. Significance was assessed using a two-tailed unpaired t-test in GraphPad Prism where ns, **, *** indicate p > 0.05, p < 0.01, and p < 0.001, respectively