Fig. 2

Alterations in NADH metabolic index of cells cultured in different media during the first hour of DOX treatment. A Upper three panels: representative intensity contrast images of MDA-MB-231 cells under HG, LG, and HBLG medium at 0 min are shown on the left and FLIM images (α1/α2) are shown towards the right in the order 0 – 60 min. Scale bar, 10 µm. Similarly, representative images of MCF-10A cells in HBLG are shown in the lower panel. B Histogram showing normalized NADH metabolic index histograms obtained from MCF-10A, MCF-7, and MDA-MB-231 in normal, LG, and HBLG medium over time. C Dot plots showing averaged NADH metabolic index obtained from MCF-10A, MCF-7, and MDA-MB-231 in normal, LG, and HBLG medium over time. Circular symbols are median values and error bars represent standard deviations over all pixels from a set of three different FOV’s per condition