Fig. 4
OXPHOShigh cell lines are smaller and exhibit less intracellular lipid storage. (A) Evaluation of electron microscopy showing a significantly increased area (µm2) of lipid droplets in OXPHOSlow (H372) and OXPHOShigh (DMS53) cell lines. (B) Lipid droplets were stained with oil red, manually counted and compared between H372 (orange bar) and DMS53 (blue bar) cells confirming the observed differenced in lipid storage. (C) Oil red staining of lipid droplets of two representative cell lines. Scale bar: 20 μm. (D) Lipid compartments in the OXPHOShigh (DMS53, H1688 and SHP77) and the OXPHOSlow cell lines (H196, H372, H841) were stained with Bodipy 493/503 and the mean fluorescence intensity (MFI) was measured via flow cytometry. Mann-Whitney tests * p ≤ 0.05, ** p < 0.01, *** p < 0.001. Data are represented as mean ± SEM. (E) Pearson correlation between cell size (x-axis) and cellular lipid content (y-axis) in OXPHOShigh (blue) and OXPHOSlow (orange) cell lines. Each dot represents the mean of one cell line. (F) Mitochondrial content measured by Mitotracker Red CXMROS (see Fig. 2B) normalized to cell size. Each dot represents the mean of one OXPHOShigh (blue) or OXPHOSlow (orange) cell line. T-Test. ** p < 0.01