Fig. 1

GPX4 and PC differentially affect TNBC cell response to redox stress. (A) Immunoblot analysis of control (shScram) and PC-knockdown (shPCB and shPCC) metM-Wnt^lung cells probed for Nrf2 and ß-Actin (n = 6/group). (B) Percent survival of metM-Wnt^lung shPC and shScramble cells treated with H₂O₂ (500µM) for 24 h (n = 4/group). (C) Top 50 Codependencies for the gene SLC7A11 from Depmap’s CRISPR dataset graphed by Pearson correlation coefficient. mRNA (D) and protein (E) expression of PC in E0771, metM-Wnt^lung, and M-Wnt cell lines treated with erastin (2.5µM) for 24 h. (D: n = 5/group, E: n = 4/group) Percent survival of E0771 (F) and metM-Wnt^lung (G) shPC and shScramble cells treated with erastin for 24 h (F: n = 5/group, G: n = 3/group). (H) Immunoblot analysis of control and PC-suppressed metM-Wnt^lung cells probed for GPX4 and ß-actin after treatment with erastin for 24 h (n = 4/group). (I) mRNA expression of PC in metM-Wnt^lung shGPX4 cells and shScramble controls (n = 5/group). Percent survival of metM-Wnt^lung (J) and E0771 (K) shPC and shScramble cells treated with RSL3 for 24 h. (J: n = 5/group, K: 4/group) Statistical significance determined by Kruskal-Wallis test with Dunn’s multiple comparison test (A, D,I); one-sample t test (E); or two-way ANOVA (B, F-H, J,K) with Tukey’s multiple comparison test (B, G), Šídák’s multiple comparison test (H), or Dunnett’s multiple comparison test (F, J,K) (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)