Fig. 1

Enhanced B16F10Luc2 and Wt31 melanoma metastasis formation after 10 weeks of CDAA diet. A Setup for metastasis formation in CDAA diet-induced MASH model after intrasplenic injection of B16F10Luc2 cells and assessment of metastatic burden 14 days later. B Macroscopic images of representative B16F10Luc2 metastatic livers of chow and CDAA groups (scale bars = 1 cm). C Counted hepatic metastasis (8 vs. 8, p = 0.0321, unpaired t-test); quantified metastatic percentage of whole liver area (5 vs. 7, p = 0.0130, unpaired t-test) and BLI measurement (photons/sec) (7 vs. 7, p = 0.0239, Mann–Whitney U test). D Total body weights (8 vs. 8, n.s., unpaired t-test), liver weights (8 vs. 8, p = 0.0193, unpaired t-test) and liver-to-body ratios (8 vs. 8, p = 0.0079, Mann–Whitney U test). E Setup for metastasis formation in CDAA diet-induced MASH model after intravenous injection (i.v.) of Wt31 cells and assessment of metastatic burden 19 days later. F Macroscopic images of representative Wt31 metastatic livers of chow and CDAA groups (scale bar = 1 cm). G Counted hepatic metastasis (6 vs. 5, p = 0.0045, Mann–Whitney U test), quantified metastatic percentage of whole liver area (6 vs. 5, p = 0.0027, unpaired t-test). H Total body weights (6 vs. 5, n.s., unpaired t-test), liver weights (6 vs. 5, p < 0.0001, unpaired t-test) and liver-to-body ratios (6 vs. 5, p = 0.0034, unpaired t-test)