Fig. 7

PI-induced PITPNM1 was required for the expression of POU1F1, C2orf15 and LDHA via the phosphorylation of AKT (pAKT) and ERK (pERK). A The effect of PITPNM1 knockdown on PI-induced the expression of POU1F1, C2orf15 and LDHA as well as the phosphorylation of AKT and ERK. Western blot analysis was used to determine the protein expression. B The effect of PITPNM1 knockdown on PI-induced migration of GH3 cells. C The effect of PITPNM1 knockdown on PI-induced invasion of GH3 cells. D The effect of PITPNM1 overexpression in the PITPNM1-depleting cells on the expression of PITPNM1, POU1F1, C2orf15 and LDHA as well as the phosphorylation of AKT and ERK. E The effect of PITPNM1 overexpression in the PITPNM1-depleting cells on the cell migration. F The effect of PITPNM1 overexpression in the PITPNM1-depleting cells on the cell invasion. G The effect of AKT activation and inhibition on PI-induced expression of PITPNM1, POU1F1, C2orf15 and LDHA. H The effect of ERK activation and inhibition on PI-induced expression of PITPNM1, POU1F1, C2orf15 and LDHA. **, p < 0.01; ***, p < 0.001