Fig. 6

The effect of phosphatidylinositol on the proliferation of invasion of pituitary adenoma cells cells and expression of PITPNM1, POU1F1 and C2orf15. A The expression of PITPNM1, POU1F1, C2orf15 and LDHA in IPA and NIPA tissues. B The primary pituitary adenoma cells (PPAC) and GH3 cells were exposed to the different concentration of phosphatidylinositol for 72 h. CCK8 assay was performed to determine the cell viability. C GH3 cells and PPAC were treated with 100 μg/ml phosphatidylinositol for indicated time. CCK8 assay was performed to determine the cell viability. D Scratch assay was performed to determine the effect of phosphatidylinositol on cell migration. E Transwell assay was performed to determine the effect of phosphatidylinositol on cell invasion. F After treatment with phosphatidylinositol, western blot analysis was performed to determine the expression of PITPNM1, POU1F1, C2orf15 and LDHA. ***, p < 0.001