Fig. 4
imCCs, but not iMEFs maintain substantial metabolic fitness following loss of SDH. (A-B) Seahorse mitochondrial stress test profiles for the indicated cell lines in 20% O2 (A) or 5% O2 (B). Rote/AA: rotenone and antimycin A. (C) Complex-specific Seahorse-based assay utilizing Complex I, II and III inhibitors or substrates to assess activities of Complexes I and II in the indicated cell lines. (D-E) Bar graphs representing data for Complex I (D) or Complex II (E) activity from panel C. Seahorse tests were performed in replicates of three (n = 3), error bars represent mean ± SEM. P-values are calculated using student’s t-test and the degree of significance is indicated with number of asterisks