Fig. 4
From: RNF2 promotes chondrosarcoma progression by regulating ubiquitination and degradation of CBX7
RNF2 inhibits CBX7 expression in CHS cells. Note: (A) UbiBrowser website to predict the downstream ubiquitination substrate of RNF2; (B) Western blot to detect CBX7 levels in clinical samples; (C) Pearson method to analyze the correlation of RNF2 and CBX7 levels in CHS samples; (D/F) RT-qPCR and Western blot (E/G) to detect CBX7 mRNA and protein expression in JJ012 cells and SW1353 cells; (H/I) Co-IP to detect the interaction between RNF2 and CBX7 in CHS cells. The cellular experiments were repeated three times, and data were expressed as mean ± standard deviation. The independent samples t-test was used for comparisons between two groups. ** P < 0.01. ns, no statistical significance