Fig. 3

Overexpression of RNF2 promotes proliferation, migration, and angiogenesis while inhibiting apoptosis in SW1353 cells. Note: pcDNA3.1-RNF2 and pcDNA3.1-NC were transfected into SW1353 cells, respectively. (A) RT-qPCR and (B) Western blot to detect RNF2 mRNA and protein expression in SW1353 cells; (C) CCK-8 to detect SW1353 cell viability; (D) EdU to detect SW1353 cell proliferation; (E) FITC/PI staining to calculate the number of early and late cell deaths for detecting SW1353 cell apoptosis; (F) Scratch assay to observe the migration distance of cells on the plate at 0 h and 24 h, respectively, for assessing SW1353 cell migratory capabilities; (G) After culture of HUVECs with conditioned media of SW1353 cells in different groups, tube formation assay to observe the number of tubules for evaluating SW1353 cell angiogenic capabilities. The cellular experiments were repeated three times, and the data were expressed as mean ± standard deviation. The independent sample t-test was used for the comparison between two groups. ** P < 0.01