Fig. 6

Susceptibility of tumor cell isolates to compounds that regulate the metabolism of amino acids and fatty acids. The cellular ATP levels in tumor cells following treatment with compounds that affect cellular metabolism were measured by the CellTiter-Glo cell viability assay. The study compounds included trimetazole (1 mM), benzylserine (10 mM), CB-839 (1 µM), etomoxir (100 µM), C75 (20 µM), orlistat (200 µM), a combination of orlistat with metformin (10 mM), ranolazine (200 µM), and triclosan (20 µM). The cells were cultivated in a complete medium (+Q, +G) or medium with depleted glucose or glutamine; the presence or absence of glucose and glutamine in the medium is indicated on the X axis. Individual data points represent the means of four measurements from each patient-derived cell isolate and are shown relative to those of the isolates in complete (control) medium (+Q, +G) without the study compound. Color codes indicate individual tumor cell isolates. The bars indicate the means and SDs