Fig. 4

Inhibition of the mitochondrial respiratory chain by high-dose metformin and xCT (SLC7A11) inhibition by erastin. The levels of resazurin reduction rates (A, C, E) and cellular ATP levels (B, D, F) following the incubation of cells with 10 mM metformin (A, B), 2 mM metformin (C, D) or 5 µM erastin (E, F). Relative absorbances of alamarBlue (A, C, E) and CellTiter-Glo (B, D, F) in tumor cells (tumor) and ovarian fibroblasts (fibroblasts) cultivated in complete medium (+Q, +G) or medium with depleted glucose (+Q, -G) or glutamine (-Q, +G). Individual data points represent the means of four measurements from each patient-derived cell isolate and are shown relative to those of the isolates in complete (control) medium (+Q, +G) without the study compound. The bars indicate the means and SDs