Fig. 2

Heatmap of the Spearman rho correlation analysis of the resazurin reduction rates and cellular ATP levels in matched tumor cells and ovarian fibroblasts from the same patient. The data are shown for the alamarBlue and CellTiter-Glo measurements analyzed as absolute values (relative to the cell count) for tumor cells (tumor) and ovarian fibroblasts (fibroblasts) cultivated in complete medium (+Q, +G) or in medium with depleted glucose (+Q, -G) or glutamine (-Q, +G). (A) Spearman rho values. (B) Spearman p values. The correlations in (A) compared data obtained from the tumors (lower right quadrant) and fibroblasts (upper left quadrant) and compared the values obtained from matched patient isolates of the two cell types (upper right quadrant). The ovals indicate significant negative correlations for which individual data points are shown in (C-E). (C-E) Dot plots of CellTiter-Glo values for cancer cells cultivated in +Q, +G medium (C), +Q, -G medium (D), and -Q, +G medium (E) compared with CellTiter-Glo values for ovarian fibroblasts from paired patient-specific samples cultivated in -Q, +G medium. The data in (C-E) were calculated relative to the number of cells present prior to the addition of the specific cell culture media