Fig. 1

Characterization of primary cell cultures. (A) Example of the FACS analysis of tumor cells (blue) and ovarian fibroblasts (red) according to their α-actin (PE) and pan-keratin (Alexa Fluor 488) expression. (B-D) Differences in the ECAR (B) and OCR (C) among tumor cells (n = 9; shown in grey) and ovarian fibroblasts (n = 2; shown in color). (D) Basal ECAR, glycolysis, glycolytic capacity, glycolytic reserve, basal respiration, proton leakage, maximum respiration, spare respiratory capacity, and non-mitochondrial respiration in tumor cells and ovarian fibroblasts. (E-H) Resazurin reduction rates and cellular ATP levels. Relative absorbances of alamarBlue (E-F) and CellTiter-Glo (G-H) in tumor cells (tumor) and ovarian fibroblasts (fibroblasts) cultivated in complete medium (+Q, +G) or medium with depleted glucose (+Q, -G) or glutamine (-Q, +G). Individual data points represent the means of four measurements from each patient-derived cell isolate and are shown relative to those of the isolates in complete medium (+Q, +G). The bars indicate the means and SDs (B, C,E, G) or SEs (D). Asterisks (^*) indicate significant differences at α < 0.05 in the REML mixed-effect model, followed by Tukey`s multiple comparison tests