Fig. 5

PAF1 overexpression increases glycolytic rate and reduces mitochondrial respiration in normal cells. A qRT-PCR analysis of PAF1, LDHA, PDK1, HK2, PDHA1 and ENO1 in HPDE vector and PAF1 OE cells, qRT-PCR data were normalized with the β-Actin gene. B Western blot analysis of PAF1 and aerobic glycolysis genes expression in PAF1 OE and Vector HPDE cells, β-actin was used as a control. C Overall ECAR curve of Vector and PAF1OE HPDE cells. Injection order: glucose (10mM), oligomycin (1 µM), and 2-DG (50 mM). The overall ECAR curves were plotted as the mean ECAR ± SD of six replicates. D Bar graph showing the glycolysis capacity, Maximal Glycolysis and Glycolytic reserve of Vector and PAF1OE HPDE cells. E Overall oxygen consumption rate (OCR) curve of Vector and PAF1OE HPDE cells. Injection order: oligomycin (1 µM), FCCP (0.5 µM), and rotenone/ antimycin A (0.5 µM). The overall OCR curves were plotted as the mean OCR ± SD of six replicates. F Bar graph showing the basal respiration, ATP-linked respiration and maximal respiration of Vector and PAF1OE HPDE cells. Data are presented as the mean ± SD (ns, not significant, **P < 0.01; ***P < 0.001; ****P < 0.0001). The representative images represent the mean ECAR and OCR ± SD of six replicates respectively