Fig. 2
From: PAF1/HIF1α axis rewires the glycolytic metabolism to fuel aggressiveness of pancreatic cancer
Co-expression of PAF1/LDHA in PC models and loss of PAF1 reduces aerobic glycolytic genes expression. A. Immunofluorescence images of SW1990, MIA PaCa-2 and HPDE cell lines stained with PAF1 and LDHA antibody. Scale bar 20 μm B. Fluorescence mean intensity quantification of LDHA and PAF1 expression in Fig. 2 (A) C. Immunofluorescence images of Human PDAC and normal pancreas tissues stained with PAF1 and LDHA antibody. Scale bar 20 μm. D. Fluorescence mean intensity quantification of LDHA and PAF1 expression in Fig. 2 (C). Quantitative real-time PCR (qRT-PCR) analysis of PAF1, LDHA, LHHB, PDK1, HK2, PDHA1, ENO1, PKFL, PKFR, HK1 in Scr and PAF1 KD (E) SW1990 and (F) MIA PaCa-2 cells, qRT-PCR data were normalized with the β-Actin gene. (G) Western blot analysis of PAF1 and aerobic glycolysis genes expression in PAF1 KD and scramble SW1990 and MiaPaCa2 cells. β-actin was used as a control. Data are presented as the mean ± SD (*p<0.05,**p<0.01, ***p<0.001)