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Fig. 2 | Cancer & Metabolism

Fig. 2

From: Epigenetic repression of de novo cysteine synthetases induces intra-cellular accumulation of cysteine in hepatocarcinoma by up-regulating the cystine uptake transporter xCT

Fig. 2

Oncogenic alterations of cysteine metabolism in murine hepatocarcinoma BNL 1ME A.7 R.1 cells. A, The protein levels of genes involved in cysteine metabolism in the liver and BNL 1ME A.7 R.1-formed tumors in mice. The protein levels were normalized to those of GAPDH (for CBS, CTH, GCLC, and GCLM) or Na+/K+ ATPase (for xCT). The values in the liver were set at 1.0. Each value represents the mean with S.D. (n = 4). *P < 0.05; significant difference between the two groups (t6 = − 3.621, P = 0.011 for GCLC; t6 = − 2.788, P = 0.032 for GCLM; t6 = 3.594, P = 0.011 for xCT; unpaired t-test, two sided). B, Intracellular contents of cysteine-related metabolites in the liver and BNL 1ME A.7 R.1-formed tumors in mice. Each value represents the mean with S.D. (n = 4–6). **P < 0.01; significant difference between the two groups (t6 = − 4.272, P = 0.005 for cystathionine; t6 = 5.171, P = 0.002 for cystine; t6 = 9.032, P = 0.001 for cysteine; t10 = − 11.398, P < 0.001 for glutathione; unpaired t-test, two sided)

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Cancer & Metabolism

ISSN: 2049-3002