Fig. 3

DHA changed the metabolites profile. Heatmap and relative quantification of assigned metabolites for (A–B) PNT1A, (C–D) 22Rv1 and (E–F) PC3 identified by ¹H-NMR after incubation with DHA at 100 µM for 48 h. Heatmaps were generated using z-score and features were clustered. Red squares mean increased concentration while blue decreased. Values in the bar graphs show the mean of relative concentration to sum and SEM (n = 6/group at least, three independent experiments) for statistically significant metabolites. Legend: NAD +—nicotinamide adenine dinucleotide; Ctrl – vehicle incubation; DHA – docosahexaenoic acid; *—statistically different from control (p < 0.05 and FDR = 0.1) after unpaired t-test