Fig. 5

NOS1 is a direct target of HIF-1α. A The consensus DNA-binding motif of HIF-1α was obtained from the JASPAR database (http://jaspar.genereg.net/). B HIF-1α Cut&Run sequencing peaks on the NOS1 gene in Hep3B cells. The putative consensus HIF-1α binding motif in the NOS1 promoter region (− 759 bp upstream of the TSS). Data were derived from the HIF-1α Cut&Run-sequencing dataset generated from Hep3B cells and downloaded from the Gene Expression Omnibus (GEO, GSE228175). C ChIP-qPCR assays on the NOS1 promoter were performed and quantified by qPCR in LOX-1 knockdown and parental HCT116 cells treated with or without oxLDL for 24 hours. D Relative luciferase activities of indicated NOS1 promoter reporters in HCT116 cells treated with or without oxLDL for 24 hours. Two-tailed Student’s t-test (C and D). Error bars represent ± SD. *p < 0.05, **p < 0.01, ***p < 0.001. Data represent mean ± SD of at least three independent experiments