Fig. 3

NO/NOS signaling is upregulated by oxLDL treatment and is needed for hypercholesterolemia-induced colorectal tumorigenesis. A Changes of total nitrite levels in oxLDL-treated HCT116 cells. B RT-qPCR analysis of NOS mRNA expression in oxLDL-treated HCT116 cells. C Correlation between LOX-1 and NOS mRNA expression in CRC samples from different CRC datasets. D The effect of oxLDL with or without L-NAME treatment on cell viability in HCT116 cells. E The effect of oxLDL with or without L-NAME treatment on clonogenic survival in HCT116 cells. F Study design of the used hypercholesterolemic ApoE^−/− mice treated with vehicle or L-NAME. G Average tumor number per mouse from vehicle- or L-NAME-treated ApoE^−/− mice. H Histopathologic stages of the tumors from vehicle- or L-NAME-treated ApoE^−/− mice. I Representative images of H&E and Ki-67 immunohistochemical staining for CRC samples from vehicle- or L-NAME-treated ApoE^−/− mice. Scale bars, 200 μm. Two-tailed Student’s t-test (A, B, D, E, G, and H), Pearson’s correlation (B). Error bars represent ± SD. ns = not significant, *p < 0.05, **p < 0.01, ***p < 0.001. Data represent mean ± SD of at least three independent experiments