Fig. 2
From: Hypoxia-induced NOS1 as a therapeutic target in hypercholesterolemia-related colorectal cancer
oxLDL/LOX-1 links hypercholesterolemia and CRC aggressiveness. A Dot plots for the mRNA expression levels of LOX-1 according to normal vs. tumor. The mRNA expression values were obtained from the TCGA-CRC dataset. B Progression-free survival according to LOX-1 mRNA expression for CRC patients from the TCGA-CRC database. C RT-qPCR analysis of LOX-1 mRNA expression in LOX-1 knockdown and parental HCT116 cells. D Western blot analysis of LOX-1 protein expression in LOX-1 knockdown and parental HCT116 cells. E The effect of oxLDL treatment on cell viability in LOX-1 knockdown and parental HCT116 cells. F The effect of oxLDL treatment on clonogenic survival in LOX-1 knockdown and parental HCT116 cells. G Study design of the used HCD-induced hypercholesterolemia murine model. H Tumor growth of shScr and shLOX-1 cells xenografted in HCD-induced hypercholesterolemia nude mice. I Tumor weight of shScr and shLOX-1 cells xenografted in HCD-induced hypercholesterolemia nude mice. J Representative images of immunohistochemistry for Ki67 staining in xenografts. Scale bars, 200 μm. Quantification of positive signals is provided on the right of the representative images. Two-tailed Student’s t-test (A, C, E, F, I and J), Log-rank test (B), One-way ANOVA (H). Error bars represent ± SD. *p < 0.05, **p < 0.01, ***p < 0.001. Data represent mean ± SD of at least three independent experiments