Fig. 1

Ketogenic diets slow the growth of melanoma xenografts independently of oncogenic driver and metabolic signature. A–D Growth curves A A375, B WM47, C WM3311, and D WM3000 melanoma xenografts treated with CTRL, LCT, or LCT-MCT diet. Mean tumor volume ± SD is shown for each group until the first CTRL mouse tumor reached the termination size; n = 10–13. See also Fig. S2. E–H. The area under the growth curve (AUC) was calculated for every mouse. AUCs are shown as individual data points ± SD; p values were determined by a one-way ANOVA with Dunnett's multiple comparisons test. I–L. Body weight of I A375, J WM47, K WM3311, and L WM3000 melanoma bearing mice during dietary intervention. Net body weight is shown as % of the initial body weight. Individual data points ± SD; n = 10–13. See also Fig. S4. M, P. Survival of M A375 and P WM3000 melanoma-bearing mice treated with CTRL, LCT or LCT-MCT (since weight loss > 20% led to the elimination of the respective animals, survival analysis based on tumor growth was not possible for the WM47 and WM3311 xenografts). LCT and LCT-MCT survival curves were compared with CTRL and p values were determined by a Log-rank (Mantel-Cox) test. N, O. Correlation analysis between tumor volume and % of initial body weight on day 14 and 22 of N WM47 and O WM3311 melanoma-bearing mice. Pearson correlation was used to compute Pearson r coefficients and the respective p values for CTRL, LCT and LCT-MCT groups separately (n = 9–10 for CTRL, n = 8–9 for LCT and n = 7–9 for LCT-MCT groups)