Fig. 6

PDHA1 supports the growth of tumor cells in media without exogenous lipids. Cell colony formation was measured in DMEM media containing either 10% complete or 10% charcoal-stripped FBS to remove non-polar compounds, in either 21% or 1% oxygen environment. Note decrease in plating efficiency in hypoxic, lipid-depleted media indicated. N = 3 biological replicates. Error bars, s.e.m. *P< 0.05, **P< 0.01, ***P< 0.0001, ****P = 0